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Applications

Applications

Applications

The ACRF Cancer Genomics Facility has expertise in a wide range of Sequencing, Microarray and related applications. Please contact the Facility (Rob King, 8222 3966, Rob.King@sa.gov.au) for more information or to enquire about any applications not listed below.

Service Sample type
Sanger Sequencing
Amplicon DNA, Plasmid DNA DNA, primers
NGS - DNA sequencing
Whole genome sequencing Genomic DNA
Whole exome sequencing Genomic DNA
Targeted DNA sequencing (pre-designed gene panels, germline and somatic) Genomic DNA
TruSight Oncology 500 panel sequencing FFPE tissue DNA and RNA
Targeted DNA sequencing (custom) Genomic DNA
ChIP-seq, ATAC-seq Prepared DNA
Long read genome sequencing (Oxford Nanopore, PacBio) Genomic DNA
Long read amplicon sequencing (Oxford Nanopore, PacBio) Amplicons
NGS - RNA sequencing
mRNA sequencing (polyA+) Total RNA
Whole transcriptome sequencing (rRNA/globin depleted) Total RNA
Small RNA (eg miRNA) sequencing Total RNA
Targeted RNA sequencing (custom) Total RNA
CLIP-seq Prepared RNA or prepared cDNA
NGS - Single Cell Sequencing
Single Cell Gene Expression (10X Genomics) Prepared cells
Single Cell Immune Profiling (10X Genomics) Prepared cells
Feature Barcoding/CITE-seq (10X/Biolegend TotalSeq applications) Prepared cells
Single Cell ATAC-seq (10X Genomics) Prepared cells
NGS – Spatial Transcriptomics
10X Genomics Visium Tissue
NGS - Sequencing only
Sequencing of client-prepared libraries Libraries (Illumina or PacBio)
Microarray services
Illumina BeadChip (eg Global Screening Array, CytoSNP arrays, MethylationEPIC) Genomic DNA
GeneChip (Affymetrix/ThermoFisher) Gene Expression Arrays (cartridge format) Total RNA
qPCR
Fluidigm Biomark qPCR analysis Total RNA
Custom Library Preparation
Please enquire  
Sample QC
DNA or RNA analysis (PerkinElmer LabChip GX, Agilent Bioanalyzer) DNA, RNA
Genomic DNA analysis (Agilent FemtoPulse) Genomic DNA
Long read library analysis (Agilent FemtoPulse) Library DNA