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Equipment

Equipment

Our extensive range of laboratory equipment and computing and data storage is available for use by the wider research community. If you have any questions about our technology or equipment please give us a call on +618 8222 3966.

High Performance Computing and data storage

We employ two entirely separate High Performance Computing environments for research and diagnostic purposes.

  • Research

This system is used for analysis of research data, as well as for R&D for both our research and diagnostic operations. Hosting, networking and security is provided by the University of Adelaide

    • 6 Intel and AMD nodes with 236 cores and a total of 2432GB RAM, 140 TB storage
    • NECTAR cloud computing
    • NCI cloud computing
  • Diagnostics

This system is used for processing of SA Pathology diagnostic samples and running NATA-approved computational genomics workflows. Hosting, networking and security is provided by SA Pathology:

      • Dell PowerEdge R740 server
      • 40 cores / 80 threads CPU
      • 384 GB memory
      • 13 TB scratch
      • 96 TB storage array

 

 Lab equipment

Type Manufacturer Model Technical Specifications
NGS Illumina 2 NextSeq 500 Benchtop sequencer, can sequence up to 120 Gb per run with 2x150bp reads in ~3 days
NGS Illumina NextSeq 550 Larger benchtop sequencer and microarray scanner, can sequence up to 120 Gb per run with 2x150bp reads in ~3 days
NGS Illumina MiSeq Fully integrated benchtop sequencer, can sequence up to 15Gb per run with 2 x 300bp reads
NGS Oxford Nanopore MinION Mk1C Single Molecule Real Time Nanopore sequencing system capable of generating reads up to 2Mb in length
NGS Pacific Biosciences Sequel Single Molecule Real Time sequencing system capable of generating reads up to 20Kb in length
Microarrays Illumina HiScan Scanner for Illumina BeadChips, include Genotyping, Linkage Analysis, Cytogenetic Analysis, Methylation and Gene Expression
Microarrays Affymetrix GeneChip Scanner & Fluidics Stations For processing SNP genotyping, cytogenetic and expression arrays
Single Cell 10X Genomics Chromium Makes RNA and multi-ome libraries from thousands of single cells.
Sanger Sequencing Applied Biosystems 3730 & 3730XL 48 and 96 capillary electrophoresis systems for fragment analysis
MALDI-TOF Agena (Sequenom) MassARRAY & Nanodispenser Highly sensitive SNP detection, can process 384 samples on a single chip
qPCR Fluidigm BioMarkHD High-throughput qPCR for gene expression and genotyping, 2304 or 9216 reactions per chip
Sample QC Agilent Bioanalyzer Digital electrophoresis system for RNA, DNA and NGS library quality control
Sample QC Perkin Elmer LabChipHT High-throughput digital electrophoresis system for RNA, DNA and NGS library quality control
Sample QC Agilent FemtoPulse Pulsed-field capillary electrophoresis system for genomic DNA analysis (up to 165kb), sensitive RNA analysis.
Sample QC Invitrogen Qubit Flex  Picogreen based assays for determining concentration of dsDNA, ssDNA and RNA
Library Prep Fluidigm Access Array High throughput PCR for generating amplicons to sequence via NGS
Library Prep Covaris ME220 DNA and chromatin shearing
Library Prep Sage Pippin Prep Automated gel-based size selection from 100bp – 1.5Kb
Library Prep Sage Blue Pippin Prep Automated gel-based size selection with Pulse-field from 100bp – 50Kb
Library Prep Perkin Elmer Sciclone NGS Workstation High-throughput liquid handler for automation of library preps, bead clean-ups and sequence capture
Library Prep Opentrons OT-2 Liquid handler for sample formatting and bead clean-ups